Rhizoactinobacteria Enhance Growth and Antioxidant Activity in Thai Jasmine Rice (Oryza sativa) KDML105 Seedlings under Salt Stress.

Salinity is one of the most devastating abiotic stresses hampering the growth and production of rice. Nine indole-3-acetic acid (IAA)-producing salt-tolerant plant-growth-promoting rhizobacteria (ST-PGPR) were inoculated into Thai jasmine rice (Oryza sativa L.) variety Khao Dawk Mali 105 (KDML105) seedlings grown under different concentrations of NaCl (0, 50, 100, and 150 mM). The ST-PGPR strains significantly promoted the growth parameters, chlorophyll content, nutrient uptake (N, P, K, Ca, and Mg), antioxidant activity, and proline accumulation in the seedlings under both normal and saline conditions compared to the respective controls. The K+/Na+ ratio of the inoculated seedlings was much higher than that of the controls, indicating greater salt tolerance. The most salt-tolerant and IAA-producing strain, Sinomonas sp. ORF15-23, yielded the highest values for all the parameters, particularly at 50 mM NaCl. The percentage increases in these parameters relative to the controls ranged from >90% to 306%. Therefore, Sinomonas sp. ORF15-23 was considered a promising ST-PGPR to be developed as a bioinoculant for enhancing the growth, salt tolerance, and aroma of KDML105 rice in salt-affected areas. Environmentally friendly technologies such as ST-PGPR bioinoculants could also support the sustainability of KDML105 geographical indication (GI) products. However, the efficiency of Sinomonas sp. ORF15-23 should be evaluated under field conditions for its effect on rice nutrient uptake and growth, including the 2AP level.

Evaluating the survival of Aspergillus niger in a highly polluted red soil with addition of Phosphogypsum and bioorganic fertilizer.

Phosphate-solubilizing fungi (PSF) can enhance P release from phosphate minerals to immobilize heavy metals. However, this promotion substantially depends on their survival in highly polluted soils. The aim of this study was to investigate the survival of PSF after addition of phosphogypsum (PG) and bioorganic fertilizer (BF) in the soil with coexistence of multiple heavy metals, e.g., Pb, As, Cd, Sb, etc. Addition of typical PSF (Aspergillus niger) did not promote the formation of pyromorphite (the most stable form of Pb), possibly due to the buffering effect of the soil (the secreted oxalic acid was neutralized) and limited P supply. Meanwhile, despite that A. niger has high tolerance to heavy metal stress, its survival was significantly declined due to the deficiency of available P. It was also shown that PG, as the major by-product in phoschemical industry, still has relatively high available P compared with common natural soils. PG addition dramatically increased available P (up to 93.87 mg/kg) and the subsequent fungal growth. However, sole PG did not promote the formation of pyromorphite, probably as the abundant Fe2+ and Mn2+ prevented the contact between PO43- and Pb2+ in the soil system. The enhanced soil respiration after addition of BF and PG confirmed the promoted microbial activity (elevated to 3465.58 µg C kg h-1). This study showed PG's potential as P source for both microbial growth and heavy metal remediation in soil system. A combination of PG, A. niger, and BF can hence achieve long-term bioremediation of heavy metals.

An investigation of the effect of operational conditions on a sequential extraction procedure for arsenic in soil in Thailand.

Sequential Extraction Procedure (SEP) can be used to evaluate the toxicity characteristics of heavy metals in soil, including arsenic (As), by separating the metals into several different fractions using selective extraction solvents in sequence. To accomplish this separation task, various factors that are known to affect the extraction process should be carefully considered. This research aimed to investigate the effect of the operational conditions on the SEP for As in soil using experimental designs. In the first step, a Plackett-Burman design was used twice to screen the important extraction variables from a total of 19 studied variables. As a result, SSR, extraction time of the first fraction (F1), and concentrations of sodium acetate (NaOAc) in F2 and ammonium oxalate (NH4Ox) in F6 were identified as significant to the amount of the extracted As. The selected variables were further investigated using a central composite design with response surface methodology. The optimized SEP characterized by 1:75 g:mL of SSR, an extraction time of 7 h 20 min of F1, 0.16 M of NaOAc and 0.11 M of NH4OAc were applied to extract a sample from contaminated agricultural soil obtained from the north of Thailand. The fractionation result was compared with the result obtained from a previously reported SEP method. It was found that similar extraction results could be achieved (91-97% As recovery). However, the optimized method revealed certain advantages in that it required dramatically less operation time (from 68 h to 32 h) and lower concentrations of the extraction solvents.